Seattle Proteome Center (SPC)
Proteomics Center at the Institute for Systems Biology

PROTOCOLS

Protocols developed by the technology development activities of the Center will be made available through publication and through this website on an ongoing basis.

Isotope Coded Affinity Tag Reagents

• Acid Cleavable ICAT Labelling Protocol
• Orig ICAT (hydrogen/deuterium) Labelling Procedure
• Protocol for Making 500 mg of Photo-Beads
• Protocol for Labelling with d0d6-GABA Photo-Beads

Isotope Coded Affinity Tag Methods

• Profiling ICAT Label Procedure for Budding Yeast Whole Proteome
• Stable Isotope Tagging of Affinity Purified Complexes

Tandem Affinity Purification

• Mammalian TAP-Tagging Technique

RNAi

• RNA interference in THP

Digestion

• Trypsin Digestion Procedures

Separation

• Cation Exchange Procedure for ICAT Samples
• Packing a Reversed-Phase Microcapillary Column